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MB Sample ID: SA000776
Local Sample ID: | 104106 |
Subject ID: | SU000030 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 0.25 years |
Gender: | male |
Animal Animal Supplier: | University of Ottawa, Canada Mary-Ellen Harper PhD, PI; |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000027 |
Sampleprep Summary: | - |
Sampleprep Protocol ID: | Mitochondrial Matrix Preparation |
Extraction Method: | Extramitochondrial Buffer Preparation: The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix. Pellets were resuspended in 100 ul of buffer (140 mM KCL, 20 HEPES, 5 K2HPO4, 1 EGTA, pH 7.4 at RT) |
Organ: | skeletal muscle |
Organ Specification: | total |
Cell Type: | myocyte |
Subcellular Location: | extramitochondrial buffer |