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MB Sample ID: SA005259
Local Sample ID: | Patient_147_FFA_A |
Subject ID: | SU000112 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | postmenopausal women |
Gender: | Female |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000108 |
Sampleprep Summary: | Metabolons standard solvent extraction method. The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for QC purposes. Sample preparation was conducted using a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by LC and one for analysis by GC. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS or GC/MS. |
Sampleprep Protocol Filename: | Metabolon_Methods_CASE-03-11VW.docx |
Sample Derivatization: | 50?L for GC/MS analysis using equal parts bistrimethyl-silyl-trifluoroacetamide and solvent mixture |