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MB Sample ID: SA012509
Local Sample ID: | 220 |
Subject ID: | SU000304 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 18-88 |
Gender: | Femal(116), Male(118) |
Human Smoking Status: | 67 nonsmokers and 167 smokers |
Human Alcohol Drug Use: | 14 no alcohol and 220 alcohol |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000312 |
Sampleprep Summary: | Frozen samples were first thawed at room temperature (25 °C) and 50 uL of each serum sample was placed in a 2 Scientific). The initial step for extraction was performed vortexed centrifuged at 20 800g for 10 min, and the supernatant was Eppendorf vial. To the first vial containing methanol was added, and the metabolite the supernatant was collected in the same vial that supernatant. The resulting supernatants dried using a Vacufuge dried mM ammonium acetate in 40% water/60% acetonitrile 5.13 ?M L-tyrosine-13C2 and 22.5 Laboratory). each through 0.45 ?m PVDF filters (Phenomenex, Torrance, analysis. A pooled sample, which was a polyp patients, and as sample and was analyzed once every 10 patient |