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MB Sample ID: SA017194
Local Sample ID: | 5Glucose5Fructose24hrReplicate1_0056.d |
Subject ID: | SU000401 |
Subject Type: | Cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000408 |
Sampleprep Summary: | Sample material was thawed on ice, vortexed for 20 s, sonicated for 5 min with a VWR 50HT Ultrasonic Bath (VWR International Inc., Bridgeport, NJ), and separated into 500 μL aliquots. Each aliquot was centrifuged for 5 min @ 14,000 rcf, and supernatant was collected and lyophilized to dryness. Samples was kept on ice and removed only for sonication, centrifugation, and lyophilization steps. Lyophilized material was used for HILIC-QTOF metabolite profiling without additional clean-up steps. Lyophilized material for GC-TOF analysis was redissolved in 1:1 acetonitrile/H2O, vortexed for 10 s, and centrifuged for 5 min @ 14,000 rcf. Supernatant was collected and lyophilized to dryness. |
Sampleprep Protocol Filename: | metabolomic_responses_of_cultured_HepG2_liver_cells.pdf |
Processing Method: | Lysophilization |