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MB Sample ID: SA019477
Local Sample ID: | Echo-A |
Subject ID: | SU000427 |
Subject Type: | Animal |
Subject Species: | Felis catus |
Taxonomy ID: | 9685 |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000434 |
Sampleprep Summary: | In all cats, Exhaled breath condensate (EBC)and Bronchoalveolar lavage fluid (BALF) and were collected before (day 0) and after (week 6) sensitization to BGA. The EBC was collected non-invasively just prior to BALF collection by placing cats in a 25 L plexiglass chamber for 20-30 min according to a previously published design with modifications [29]. BALF was collected in a blind fashion under anesthesia using an 8 French red rubber catheter passed through an endotracheal tube according to a previously described protocol [30]. The percentage of eosinophils in each BALF modified Wright’s stained cytospin was determined by counting 200 nucleated cells, with an asthmatic phenotype defined as >17% eosinophils.The EBC and the supernatant of centrifuged BALF remaining were promptly stored at -80 °C until further analysis. NMR spectra of the EBC were measured for use in training and evaluating statistical approaches and biomarkers. |