Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA020082

Local Sample ID:	B_75
Subject ID:	SU000431
Subject Type:	Mice
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

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Sample Preparation:

Sampleprep ID:	SP000438
Sampleprep Summary:	The NMR samples were used for the neurotransmitter analysis after NMR data acquisition. These samples were lyophilized overnight followed by reconstitution in 50 µL 95:5 methanol:water. Following a final centrifugation, extracts were transferred to vials for analysis by injection (20 µL) onto the Thermo Scientific (TS) 3000 series U-HPLC system. Compound separation was achieved on a Thermo Scientific Hypersil Gold, 200 x 2.1 mm column, and 1.9 µm particle size with an isocratic flow rate of 0.400 mL/min with a column temperature of 30°C. Simultaneous detection of neurotransmitters was achieved by the TS 5600A 16 channel coulometric array ECD with coulometric cells set at incremental potentials ranging from -150 mV to 900 mV. A Standard Mix containing 14 target compounds at known concentrations was analyzed just prior to each batch to confirm the retention time and elution channel of each compound. Additionally, the mix was used as an external standard to semi-quantitate any targets found in the extracts.

Sampleprep ID:

SP000438

Sampleprep Summary:

The NMR samples were used for the neurotransmitter analysis after NMR data acquisition. These samples were lyophilized overnight followed by reconstitution in 50 µL 95:5 methanol:water. Following a final centrifugation, extracts were transferred to vials for analysis by injection (20 µL) onto the Thermo Scientific (TS) 3000 series U-HPLC system. Compound separation was achieved on a Thermo Scientific Hypersil Gold, 200 x 2.1 mm column, and 1.9 µm particle size with an isocratic flow rate of 0.400 mL/min with a column temperature of 30°C. Simultaneous detection of neurotransmitters was achieved by the TS 5600A 16 channel coulometric array ECD with coulometric cells set at incremental potentials ranging from -150 mV to 900 mV. A Standard Mix containing 14 target compounds at known concentrations was analyzed just prior to each batch to confirm the retention time and elution channel of each compound. Additionally, the mix was used as an external standard to semi-quantitate any targets found in the extracts.