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MB Sample ID: SA025754
Local Sample ID: | ML-12 |
Subject ID: | SU000518 |
Subject Type: | LC-MS & GC-MS |
Subject Species: | Nicotiana tabacum |
Taxonomy ID: | 4097 |
Species Group: | Plant |
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Sample Preparation:
Sampleprep ID: | SP000525 |
Sampleprep Summary: | Samples were prepared using the automated MicroLab STAR® system (Hamilton Company, Salt Lake City, UT). A 6-mg quantity of each lyophilized leaf sample was thawed on ice and extracted using the automated MicroLab STAR system in 400 L of methanol (LC-MS Ultra CHROMASOLV®, Riedel-de Haёn) containing recovery standards. The samples were prepared using a proprietary series of organic and aqueous extractions to remove the protein fraction while maximizing the recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by liquid chromatography (LC) and one for analysis by gas chromatography (GC). The samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum, followed by preparation for either LC/MS or GC/MS. |