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MB Sample ID: SA027267
| Local Sample ID: | 20150724_YU_1_1_14 |
| Subject ID: | SU000544 |
| Subject Type: | Animal |
| Subject Species: | Megalobrama amblycephala |
| Taxonomy ID: | 75352 |
| Species Group: | Fish |
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Sample Preparation:
| Sampleprep ID: | SP000551 |
| Sampleprep Summary: | To initiate the experiment, each plasma sample was mixed with the same volume and labeled as Quality control (QC) sample. Plasma sample (100 µl, including QC sample) was added into new Eppendorf tube with ice-cold methanol (300 µl), vortex mixing for 1 min, standing for 1 min, and make sure the solution volume had no obvious observable difference. Then samples were precipitated for 20 min at -20°C, and centrifuged at 14000 rpm for 20 min at 4°C to precipitate the proteins. A total of 250 µl of protein free supernatant was collected and dried with nitrogen at 37°C. The concentration of extractant was diluted depending on the mass spectrum response signals. |
| Sampleprep Protocol Filename: | Zhou999_20161213_161728_PR_SP_sampleprep.docx |
