Return to study ST000603 main page
MB Sample ID: SA033296
| Local Sample ID: | 160427blvsa12_1 |
| Subject ID: | SU000626 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000633 |
| Sampleprep Summary: | 1. Vortex the urine samples for about 10s to obtain a homogenized sample using the MiniVortexer VWR. 2. Aliquot 15-30ul and add 1ml extraction solution. The extraction solution has to be pre-chilled using the ThermoElectron Neslab RTE 740 cooling bath set to -20°C. 3. Vortex the sample for about 10s and shake for 5min at 4°C using the Orbital Mixing Chilling/Heating plate. If you are using more than one sample, keep the rest of the samples on ice (chilled at <0°C with NaCl). 4. Centrifuge samples for 2min at 14000 rcf using the centrifuge Eppendorf 5415 D. 5. Aliquot 2X 0.5ml supernatant. One for analysis and one for backup. Store 2nd aliquot in -20°C freezer. 6. Evaporate 0.5ml aliquots of the sample in the Labconco Centrivap cold trap concentrator to complete dryness. 7. Submit to derivatization. |
| Sampleprep Protocol Filename: | SOP_Extraction_of_Urine.pdf |
