Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA034994

Local Sample ID:	12M_MTM
Subject ID:	SU000643
Subject Type:	Mouse
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP000650
Sampleprep Summary:	AH and TM samples were subjected to lipid extraction using suitable modification of the Bligh and Dryer method. All extractions were made without addition of any extraneous ions such as lithium or acetate ions unless stated otherwise. Some confirmatory additional experiments were performed, where 1mM LiCl or 1mM ammonium acetate (for positive and negative ion mode analyses, respectively) was added to the aliquot being analyzed just before analyses. The samples were dried with a Speed-Vac and flushed with argon gas. Uniformity of extraction was ensured with an added external standard (1, 2-ditridecanoyl-snglycero-3-phosphocholine) during tissue homogenization. All extractions and subsequent handling were done using glass vials to avoid contaminating impurities. Extracted lipids were dried and resuspended in LC-MS grade Acetonitrile:Isopropanol (1:1).

Sampleprep ID:

SP000650

Sampleprep Summary:

AH and TM samples were subjected to lipid extraction using suitable modification of the Bligh and Dryer method. All extractions were made without addition of any extraneous ions such as lithium or acetate ions unless stated otherwise. Some confirmatory additional experiments were performed, where 1mM LiCl or 1mM ammonium acetate (for positive and negative ion mode analyses, respectively) was added to the aliquot being analyzed just before analyses. The samples were dried with a Speed-Vac and flushed with argon gas. Uniformity of extraction was ensured with an added external standard (1, 2-ditridecanoyl-snglycero-3-phosphocholine) during tissue homogenization. All extractions and subsequent handling were done using glass vials to avoid contaminating impurities. Extracted lipids were dried and resuspended in LC-MS grade Acetonitrile:Isopropanol (1:1).