Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA042056

Local Sample ID:	03_CBS_3
Subject ID:	SU000789
Subject Type:	plant
Subject Species:	Glycine max
Taxonomy ID:	3847
Species Group:	Plant

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Sample Preparation
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MS

Sample Preparation:

Sampleprep ID:	SP000796
Sampleprep Summary:	Dried black soybeans were ground into a fine powder and sieved through a screen with a 55 mesh count, then 40 mg of the powder was mixed with 70% aqueous methanol (4mL, 1% HCl) containing 1.5 μg/ml phlorizin as an internal standard and sonicated for 30 min. The mixture was kept for approximately 16 h at room temperature in the dark.vacuum.Supernatants were filtered through a 0.20-μm syringe filter and freeze dried then reconstituted with 0.5 ml of 50% aqueous methanol for LC-MS analysis.

Sampleprep ID:

SP000796

Sampleprep Summary:

Dried black soybeans were ground into a fine powder and sieved through a screen with a 55 mesh count, then 40 mg of the powder was mixed with 70% aqueous methanol (4mL, 1% HCl) containing 1.5 μg/ml phlorizin as an internal standard and sonicated for 30 min. The mixture was kept for approximately 16 h at room temperature in the dark.vacuum.Supernatants were filtered through a 0.20-μm syringe filter and freeze dried then reconstituted with 0.5 ml of 50% aqueous methanol for LC-MS analysis.