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MB Sample ID: SA051611
Local Sample ID: | 221_PM_24HRPOST |
Subject ID: | SU000923 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Weight Or Weight Range: | Normal/Overweight/Obese |
Gender: | Male/Female |
Human Race: | Black/White |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000930 |
Sampleprep Summary: | Aliquots of 30µL plasma were mixed with 1mL 3:3:2 ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |