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MB Sample ID: SA054091
Local Sample ID: | BCP12 |
Subject ID: | SU000956 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 35 - 65 |
Weight Or Weight Range: | 61 - 75 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP000963 |
Sampleprep Summary: | 40 µL of plasma was deproteinized with cold acetonitrile (1:3,−20 °C), followed by a two-step derivatization: (i) methoximation with O-Methoxyamine hydrochloride in pyridine (15 mg/mL, room temperature, 16 h) followed by (ii) silylation with BSTFA containing 1% TMCS (70 °C, 1 h). |
Processing Method: | Protein precipitation followed by metabolite extraction |
Processing Storage Conditions: | On ice |
Extraction Method: | Acetonitrile |
Sample Derivatization: | Methoximation and silylation |