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MB Sample ID: SA069459
| Local Sample ID: | 82_FC_15 |
| Subject ID: | SU001077 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Human Inclusion Criteria: | Caesarean delivery, Non-labored, Full term |
| Human Exclusion Criteria: | Twins |
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Sample Preparation:
| Sampleprep ID: | SP001084 |
| Sampleprep Summary: | The placental tissue samples (24.15–192.21 mg; mean 126.21 mg) were cut on dry ice and washed with ice-cold 0.85% sodium chloride to remove the excess blood before being placed in prechilled tubes. Five hundred μL of ice-cold 50/50 methanol/water was added to each sample before vortexing for 1 min. Homogenization and sonication methods were compared for metabolite extractions. Equivalent results were observed for both methods and, therefore, sonication was used because of its ability for high-throughput sample preparation. The samples were sonicated for 20 min to extract the metabolites and then spun at 14,000 rcf for 15 min at 4 °C. Four hundred and fifty μL of supernatant was transferred to a new microcentrifuge tube and concentrated overnight using a CentriVap Benchtop Vacuum Concentrator (Labconco, Kanas City, MO, USA). The samples were frozen at −80 °C until metabolomics analysis. he concentrated tissue specimens were thawed, reconstituted in 600 μL of 100 mM sodium phosphate buffer at pH 7.0, and vortexed until the pellets dissolved. The samples were centrifuged at 14,000 rcf for 15 min at 4 °C before transferring 590 μL into 5 mm NMR tubes (Bruker Biospin, USA). Organ: Placenta, Maternal and fetal surfaces |
| Processing Method: | Sonication and solvent removal w/Speed Vac |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 1:1 MeOH/H2O |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 600uL of 100mM sodium phosphate buffer at pH of 7.0 |
