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MB Sample ID: SA072413
Local Sample ID: | QC6 |
Subject ID: | SU001117 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Tcf/LacZ mice |
Age Or Age Range: | 6-8 weeks |
Gender: | Male and female |
Animal Inclusion Criteria: | The Tcf/LacZ strain was previously backcrossed onto the C3H background C3H background, and confirmed by PCR to lack the rd1 mutation |
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Sample Preparation:
Sampleprep ID: | SP001124 |
Sampleprep Summary: | 4 mL of methanol (LC-MS grade) and 2 mL of chloroform (LC-MS grade) were added to each sample. After 2 min vigorous vortexing and 2 min sonication in ultrasonic bath, the samples were incubated at 48˚C overnight (in borosilicate glass vials, PTFE-lined caps). The following day, 2 mL of water (LC-MS grade) and 1 mL of chloroform were added, samples vigorously vortexed for 2 min and centrifuged at 3000 RCF, 4˚C for 15 min to obtain phase separation. Lower phases were collected and dried in centrifugal vacuum concentrator. Samples were stored at -20˚C until reconstituted in 150 µL of chloroform:methanol (1:1) prior to mass spectrometric analysis. Lipid profiling was performed in 2 batches: 7 day and 15 day period. For the 15 day period, quality control (QC) pooled sample was prepared and run 6 times throughout the batch. |
Processing Method: | lipid extraction |
Processing Storage Conditions: | Described in summary |
Extraction Method: | chloroform, methanol, water phase separation |
Extract Storage: | -80℃ |
Sample Resuspension: | 150 µL of chloroform:methanol (1:1) |
Sample Spiking: | no internal standards added |