Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA073595

Local Sample ID:	20
Subject ID:	SU001133
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	B6Ly5.2

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Sample Preparation:

Sampleprep ID:	SP001140
Sampleprep Summary:	To determine targeted taurine quantitation, samples (int estinal fecal content) from mice 21d post-transplant were harvested, homogenized, and snap-frozen in liquid N2. Fecal content was we ighed at necropsy. Samples were extracted with acetonitrile spiked with stable isotope-labeled C13 taurine standards. The supernatan t was analyzed by Agilent 6490 mass spectrometer in a positive electrospray ionization mode after liquid chromatography using a HILI C mode and isocratic gradient. Quantitation was performed by calibration to internal standards and expressed in mM after normalizati on for weights.

Sampleprep ID:

SP001140

Sampleprep Summary:

To determine targeted taurine quantitation, samples (int estinal fecal content) from mice 21d post-transplant were harvested, homogenized, and snap-frozen in liquid N2. Fecal content was we ighed at necropsy. Samples were extracted with acetonitrile spiked with stable isotope-labeled C13 taurine standards. The supernatan t was analyzed by Agilent 6490 mass spectrometer in a positive electrospray ionization mode after liquid chromatography using a HILI C mode and isocratic gradient. Quantitation was performed by calibration to internal standards and expressed in mM after normalizati on for weights.