Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA075880

Local Sample ID:	M-GroupII_37
Subject ID:	SU001157
Subject Type:	Fish
Subject Species:	Megalobrama amblycephala
Taxonomy ID:	75352

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Sample Preparation:

Sampleprep ID:	SP001164
Sampleprep Summary:	Muscle tissues about 25 mg from each individual were homogenized in 800 µL cold methanol and water (v/v = 1:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). 400 µL supernatant was collected after centrifugation (25000 g, 4 °C, 10 min). The supernatant in the eppendorf tube was removed and the sediment in the tube was dried. Homogenized the sediment again in 800 µL cold dichloromethane and methanol (v/v = 3:1), after centrifuging and then collected 200 µL supernatant for QC (quality control) sample (25000 g, 4 °C, 10 min). The supernatant was evacuated under vacuum, re-collected 100µL supernatant after centrifugation (25000 g, 4 °C, 10 min) for subsequent detection.

Sampleprep ID:

SP001164

Sampleprep Summary:

Muscle tissues about 25 mg from each individual were homogenized in 800 µL cold methanol and water (v/v = 1:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). 400 µL supernatant was collected after centrifugation (25000 g, 4 °C, 10 min). The supernatant in the eppendorf tube was removed and the sediment in the tube was dried. Homogenized the sediment again in 800 µL cold dichloromethane and methanol (v/v = 3:1), after centrifuging and then collected 200 µL supernatant for QC (quality control) sample (25000 g, 4 °C, 10 min). The supernatant was evacuated under vacuum, re-collected 100µL supernatant after centrifugation (25000 g, 4 °C, 10 min) for subsequent detection.