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MB Sample ID: SA080352
Local Sample ID: | JHOP-05727 |
Subject ID: | SU001223 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP001231 |
Sampleprep Summary: | Samples were prepared using the automated MicroLab STARĀ® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVapĀ® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis. |
Sampleprep Protocol Filename: | Sample_Preparation_Pluznick.pdf |