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MB Sample ID: SA082719
Local Sample ID: | NEG S-2 |
Subject ID: | SU001258 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP001266 |
Sampleprep Summary: | Total metabolites was extracted from the per group cell (~1x10e7) using 1 ml of methanolic acetonitrile aqueous solution (2:2:1, v/v), vortex for 60 s, hypothermia for 30 min, 2 times, place the protein for 1 h at -20 ° C, centrifuge at 14000 rcf for 20 min at 4 ° C, and freeze the supernatant. -80 ° C to save the sample |