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MB Sample ID: SA086161
Local Sample ID: | p_rqc3 |
Subject ID: | SU001285 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 24 weeks |
Weight Or Weight Range: | 20-30 gram |
Gender: | Male |
Animal Housing: | Research Animal Resource Center of Weill Cornell Medicine |
Animal Feed: | regular chow (#88406, Harlan, Madison, WI) or VA-deficient diet (#88407, Harlan) |
Animal Water: | regular water |
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Sample Preparation:
Sampleprep ID: | SP001293 |
Sampleprep Summary: | After washes in cold PBS, samples were lysed by three cycles of bead-beating in 80% -70 ᵒC methanol: water using a tissue lyser cell disrupter. Proteins were removed from the extraction mixture by centrifugation to yield the metabolites for analysis. The metabolites were dried in a speed-vac, and stored at -80 ᵒC. Prior to measurement, the metabolites were solubilized in 0.2 M NaOH and subsequently analyzed by LC/MS and LC MS/MS |