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MB Sample ID: SA086295
| Local Sample ID: | EL-NYMC-6-Run 1 |
| Subject ID: | SU001290 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP001298 |
| Sampleprep Summary: | Small molecule metabolites were extracted from sera and analyzed by LC-MS as previously described. Specifically, cold methanol (60 µl) was added to 20 µl of sera, vortexed and incubated at -20°C for 1 h. Samples were warmed to room temperature for ten minutes, vortexed and centrifuged at 18,000 x g for 30 min. The supernatant (65 µl) was transferred to a new microcentrifuge tube and dried under vacuum. The dried metabolite extract was suspended in 50% methanol (40 µl) centrifuged at 18,000 x g for 15 min. An aliquot (35 µl) was transferred to an autosampler vial for LC-MS analysis. |
| Sampleprep Protocol Comments: | doi:10.1038/nprot.2011.335 |
