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MB Sample ID: SA091387
| Local Sample ID: | S31-105 Pre-Po |
| Subject ID: | SU001325 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
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Sample Preparation:
| Sampleprep ID: | SP001333 |
| Sampleprep Summary: | Individual food and urine samples were stored at -80 °C prior to sample preparation. Individual foods (including apple juice) were individually dried using a FreeZone 2.5 plus lyophilizer (Labconco, Kansas City, MO, USA). Dried samples were then divided and macerated using a chilled bio-pulverizer (BioSpec Products, Bartlesville, OK). Approximately 50 mg of each sample was measured into chilled microcentrifuge tubes and stored at -80o C prior to further processes and analysis. 10 µL of coffee was diluted with 90 µL of water, vortexed, aliquotted and stored at -80° C until analysis. An aliquot of coffee was also reserved for neat (i.e. unprocessed) analysis, and stored at -80° C until analysis. Freeze-dried foods were removed from -80° C and allowed to thaw on ice. To each sample, 480 μl of chilled methanol, 10 µl each of a hydrophilic spike mix, hydrophilic positive control mix, hydrophobic spike mix and hydrophobic positive control mix were added (see Supplemental Table S1). Samples were gently vortexed for 10 seconds, placed at -80o C for 60 minutes, and then centrifuged 0o C at 18,000 x g for 15 minutes to facilitate protein precipitation. Supernatants were transferred to new microcentrifuge tubes and dried using vacuum centrifugation at 45o C for approximately 60 minutes. Each sample was suspended in 50 µl of 95:5 LC/MS grade water-acetonitrile and gently vortexed for 30 seconds. Following a quick centrifugation, each sample was then divided into replicate 20 µl aliquots, while 10 µl was removed from each sample to generate a pooled QC sample. Samples were stored at -80° C until analysis. Urine samples were analyzed neat (i.e. without any preparation) except that authentic standards were spiked into urine samples to monitor variability in instrument response or batch effects. |
| Processing Storage Conditions: | Described in summary |
