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MB Sample ID: SA093735
Local Sample ID: | SG222 |
Subject ID: | SU001362 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | SmoA1-Math-GFP |
Age Or Age Range: | 12-36 weeks |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP001370 |
Sampleprep Summary: | Frozen mice brain tissue samples were cryo-pulverized with liquid nitrogen and weighed. 2-propanol (20 uL/mg) was added to the cryo-pulverized tissue samples to precipitate proteins. Samples were vortex-mixed for 1 min, and centrifuged at 13,000 rpm for 7 min. Following centrifugation, 100 uL of supernatant was transferred to an autosampler vial and analyzed with UPLC-MS. A blank sample, prepared with 100 uL 2-propanol, underwent the same process as the murine tumor samples. A pooled quality control (QC) sample was prepared by mixing a 10 uL aliquot of supernatant of each sample. |