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MB Sample ID: SA097881
| Local Sample ID: | AR404_control_AA_GFP_2pt1 |
| Subject ID: | SU001422 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6JMarp |
| Age Or Age Range: | 7 to 12 weeks |
| Gender: | Male |
| Animal Animal Supplier: | Monash University Animal Research Platform, Clayton, AUS |
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Sample Preparation:
| Sampleprep ID: | SP001430 |
| Sampleprep Summary: | Livers were cryogenically pulverized (cryopulverization) using a 12-well biopulverizer (BioSpec Products, OK USA Part number 59012MS) according to the manufacturer’s instructions. The frozen tissue powder was then weighed and extracted in 20 µL of extraction solvent (0°C) per mg of tissue. The mixture was then briefly vortexed before sonication in an ice-water bath for 10 minutes followed by centrifugation (20,000 rcf, 4℃, 10 minutes). The supernatant was then transferred to a mass spectrometry vial for LC-MS analysis. The extraction solvent consisted of 2:6:1 CHCl3:MeOH:H2O v/v/v with 2 µM CHAPS, CAPS, PIPES and TRIS as internal standards. Additionally where quantitative amino acid analysis was performed, a mixture of 17 stable isotope labelled amino acids (Gln, Asn and Trp were absent) were added at a concentration of 500 pmol of each amino acid per mg liver (Cambridge Isotope Laboratories PN MSK-A2-1.2). |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | -80℃ |
