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MB Sample ID: SA099803
| Local Sample ID: | BOL1_POS C |
| Subject ID: | SU001445 |
| Subject Type: | Plant |
| Subject Species: | Quercus ilex |
| Taxonomy ID: | 58334 |
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Sample Preparation:
| Sampleprep ID: | SP001453 |
| Sampleprep Summary: | Metabolites were extracted from acorn flour as described by Valledor et al., (2014), with minor modifications. 600 µL of ice-cold methanol: chloroform: water (5:2:2) was added to 50 mg of acorn flour, mixed by vortexing, and the mixture sonicated (ultrasonic bath, 40 kHZ for 10 min). After centrifugation (4 oC, 4 min, 20,000 × g), the pellet was once more extracted with 200 µL of cold methanol: chloroform: water (5:2:2). The two supernatants were combined and vacuum dried at 30 oC (Speedvac, Eppendorf Vacuum Concentrator Plus/5301). Dried extracts were reconstituted in methanol, centrifuged at 20,000 × g for 10 min, filtered through 0.22 µm PTPE membranes (Thermo Scientific, MA, USA) and filtrate collected in 1.5 mL LC/MS certified sample vials. |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | On ice |
| Sample Resuspension: | Methanol |
