Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA114480

Local Sample ID:	PL24_HA_40151
Subject ID:	SU001482
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP001490
Sampleprep Summary:	Plasma metabolites were extracted from pre-aliquoted EDTA plasma (10 µL) with 30µL of LCMS grade methanol (ThermoFisher) in a 96-well microplate (Eppendorf). Plates were heat sealed, vortexed for 5 min at 750 rpm, and centrifuged at 2000 × g for 10 minutes at room temperature. The supernatant (10 µL) was carefully transferred to a 96-well plate, leaving behind the precipitated protein. The supernatant was further diluted with 10 µL of 100 mM ammonium formate, pH3. For Hydrophilic Interaction Liquid Chromatography (HILIC) analysis, the samples were diluted with 60 µL LCMS grade acetonitrile (ThermoFisher), whereas samples for C18 analysis were diluted with 60 µL water (GenPure ultrapure water system, Thermofisher). Each sample solution was transferred to 384-well microplate (Eppendorf) for LCMS analysis. For lipidomics, Pre-aliquoted EDTA plasma samples (10 µL) were extracted with 30µL of LCMS grade 2-propanol (ThermoFisher) in a 96-well microplate (Eppendorf). Plates were heat sealed, vortexed for 5min at 750 rpm, and centrifuged at 2000 x g for 10 minutes at room temperature. The supernatant (10µL) was carefully transferred to a 96-well plate, leaving behind the precipitated protein. The supernatant was further diluted with 90µL of 1:3:2 100mM ammonium formate, pH3 (Fischer Scientific): acetonitrile: 2-propanol and transferred to a 384-well microplate (Eppendorf) for lipids analysis using LCMS.

Sampleprep ID:

SP001490

Sampleprep Summary:

Plasma metabolites were extracted from pre-aliquoted EDTA plasma (10 µL) with 30µL of LCMS grade methanol (ThermoFisher) in a 96-well microplate (Eppendorf). Plates were heat sealed, vortexed for 5 min at 750 rpm, and centrifuged at 2000 × g for 10 minutes at room temperature. The supernatant (10 µL) was carefully transferred to a 96-well plate, leaving behind the precipitated protein. The supernatant was further diluted with 10 µL of 100 mM ammonium formate, pH3. For Hydrophilic Interaction Liquid Chromatography (HILIC) analysis, the samples were diluted with 60 µL LCMS grade acetonitrile (ThermoFisher), whereas samples for C18 analysis were diluted with 60 µL water (GenPure ultrapure water system, Thermofisher). Each sample solution was transferred to 384-well microplate (Eppendorf) for LCMS analysis. For lipidomics, Pre-aliquoted EDTA plasma samples (10 µL) were extracted with 30µL of LCMS grade 2-propanol (ThermoFisher) in a 96-well microplate (Eppendorf). Plates were heat sealed, vortexed for 5min at 750 rpm, and centrifuged at 2000 x g for 10 minutes at room temperature. The supernatant (10µL) was carefully transferred to a 96-well plate, leaving behind the precipitated protein. The supernatant was further diluted with 90µL of 1:3:2 100mM ammonium formate, pH3 (Fischer Scientific): acetonitrile: 2-propanol and transferred to a 384-well microplate (Eppendorf) for lipids analysis using LCMS.