Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA120818

Local Sample ID:	657
Subject ID:	SU001504
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Female

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Sample Preparation:

Sampleprep ID:	SP001512
Sampleprep Summary:	784 normal pregnancy samples (3 outlier samples were removed) were completely randomized within each cohort (Discovery and Validation - 1) and analyzed in 12 batches across two years. 200 μL plasma was extracted by mixing 800 μL 1:1:1 acetone: acetonitrile: methanol with the internal standard mixture. The extraction mixture was vortexed and mixed for 15 min at 4 C and incubated at -20 C for 2 hours to allow protein precipitation. The supernatant was collected after centrifugation and evaporated to dryness under nitrogen (Biotage Turbovap). The dry extracts were reconstituted with 200 μL 1:1 methanol: water before analysis.

Sampleprep ID:

SP001512

Sampleprep Summary:

784 normal pregnancy samples (3 outlier samples were removed) were completely randomized within each cohort (Discovery and Validation - 1) and analyzed in 12 batches across two years. 200 μL plasma was extracted by mixing 800 μL 1:1:1 acetone: acetonitrile: methanol with the internal standard mixture. The extraction mixture was vortexed and mixed for 15 min at 4 C and incubated at -20 C for 2 hours to allow protein precipitation. The supernatant was collected after centrifugation and evaporated to dryness under nitrogen (Biotage Turbovap). The dry extracts were reconstituted with 200 μL 1:1 methanol: water before analysis.