Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA121901

Local Sample ID:	QC_04
Subject ID:	SU001508
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation:

Sampleprep ID:	SP001516
Sampleprep Summary:	Frozen liver tissues were lysed in 0.5X PBS (sample:solvent 1:10 ratio) containing 1 mg/mL butylhydroxytoluene (BHT) solution in methanol (proportion 200 µL per g of tissue), with FastPrep-24 5G instrument (MP Biomedicals, USA). 25 µL aliquots of homogenate were collected and stored at -80°C until lipidomic extraction. Tissue homogenates were thawed and extracted with methyl-tert-butylether (MTBE) as described in [Matyash, V., et al., Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. Journal of lipid research, 2008. 49(5): p. 1137-46]. 900 µL of organic phase were dried-out in speedvac and resuspended in 50 µL of ACN:H2O (20:80, v:v) just before injection.

Sampleprep ID:

SP001516

Sampleprep Summary:

Frozen liver tissues were lysed in 0.5X PBS (sample:solvent 1:10 ratio) containing 1 mg/mL butylhydroxytoluene (BHT) solution in methanol (proportion 200 µL per g of tissue), with FastPrep-24 5G instrument (MP Biomedicals, USA). 25 µL aliquots of homogenate were collected and stored at -80°C until lipidomic extraction. Tissue homogenates were thawed and extracted with methyl-tert-butylether (MTBE) as described in [Matyash, V., et al., Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. Journal of lipid research, 2008. 49(5): p. 1137-46]. 900 µL of organic phase were dried-out in speedvac and resuspended in 50 µL of ACN:H2O (20:80, v:v) just before injection.