Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA122014

Local Sample ID:	Sample_02
Subject ID:	SU001510
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	C57Bl/6
Age Or Age Range:	10-20 weeks
Gender:	Male

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MS

Sample Preparation:

Sampleprep ID:	SP001518
Sampleprep Summary:	Metabolites were extracted with 4 volumes of cold methanol/acetonitrile (1:1, v/v). To precipitate proteins, the samples were incubated for 1h at -20°C. After the samples were centrifuged at 4°C for 15 min at 13,000 rpm, the supernatant was collected and evaporated to dryness in a vacuum concentrator. The dry extracts were then reconstituted in 90 μL of acetonitrile/H2O (1:1, v/v) containing 10 ng/mL CUDA (12-[[(cyclohexylamino)carbonyl] amino]-dodecanoic acid).

Sampleprep ID:

SP001518

Sampleprep Summary:

Metabolites were extracted with 4 volumes of cold methanol/acetonitrile (1:1, v/v). To precipitate proteins, the samples were incubated for 1h at -20°C. After the samples were centrifuged at 4°C for 15 min at 13,000 rpm, the supernatant was collected and evaporated to dryness in a vacuum concentrator. The dry extracts were then reconstituted in 90 μL of acetonitrile/H2O (1:1, v/v) containing 10 ng/mL CUDA (12-[[(cyclohexylamino)carbonyl] amino]-dodecanoic acid).