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MB Sample ID: SA125861
Local Sample ID: | C1 |
Subject ID: | SU001566 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Fischer 344 |
Age Or Age Range: | 6 wk |
Gender: | Female |
Animal Animal Supplier: | Envigo |
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Sample Preparation:
Sampleprep ID: | SP001574 |
Sampleprep Summary: | NSC seeded gels were snap frozen and stored at -80C until extraction. Two gels were combined per group and extracted using a modified Bligh and Dyer extraction technique. In brief, 100 uL of methanol was added to each sample then underwent a series of snap freezing, sonication and vortexing three times. 750 μl of 1:2 chloroform:methanol was added to each homogenized sample, vortexed, then an additional 250 μL of chloroform was added, finally 250 μL water was added, all solvents used were LC grade. Samples were stored in -20 overnight and centrifuged to separate the phase layers and solidify the protein precipitate interface. Aqueous and organic layers were separated, dried down using Centrivap (Labconco) and stored in the -80 C until use. Aqueous portions of the extract were resuspended in 200 μL of 35% acetonitrile. |