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MB Sample ID: SA126030
Local Sample ID: | SIHUMI_Bw_Tau_1_A |
Subject ID: | SU001570 |
Subject Type: | Bacteria |
Subject Species: | SIHUMI consortia |
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Sample Preparation:
Sampleprep ID: | SP001578 |
Sampleprep Summary: | For the analysis of sulfonates by LC-MS/MS-MRM, aliquots of 250 µl for each sample were centrifuged (14 000 x g, 4 °C, 5 min) and 50 µl of the supernatant stored at – 20 °C until further processing. Samples were thawed, centrifuged at 18 000 x g at RT for 2 min and the supernatant was diluted 1:10 000 in 50% aqueous acetonitrile. Subsequently, 500 µl of each sample were placed in glass vials (Wicom, Heppenheim, Germany) and stored at - 80 °C until LC-MS/MS analysis. |
Sampleprep Protocol Filename: | sven_haange_Sample_preparation-application_experiment.pdf |