Return to study ST001527 main page
MB Sample ID: SA128713
Local Sample ID: | CDSR01405-01_neg |
Subject ID: | SU001601 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001609 |
Sampleprep Summary: | After incubation, media and tissue sample were transferred to 1.5mL microcentrifuge tube and centrifuged for 5min. 13C-glucose media was aspirated and specimen was washed with PBS and centrifuged for 5min, twice. After wash, 500mL acetonitrile was added and tissue was homogenized with pellet mixer. After 2-3min homogenization, 376mL of DNase/RNase free water and 250mL chloroform were added. Contents were vortexed until milky-white color and centrifuged for 20min. Top (polar) layer was aspirated and frozen at -80oC. As control, a NSCLC tissue biopsy was incubated in unlabeled glucose media for 24h and processed likewise. Sample polar layers were flash frozen in liquid N2, then lyophilized for 24-48h until dried |