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MB Sample ID: SA130702
Local Sample ID: | M_isoprenaline_3 |
Subject ID: | SU001625 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP001631 |
Sampleprep Summary: | Cells were extracted as previously described (14). Briefly, cells were quenched and washed three times with 4 °C Dulbecco’s phosphate buffered saline (DPBS; Invitrogen), then scraped in 750 µL of ice-cold extraction solvent (chloroform: methanol: water = 1:3:1). After scraping, the samples were mixed thoroughly on a vortex mixer for 30 min at 1200 rpm (4 °C) and centrifuged at 20,000 g for 10 min (4 °C). Samples were then evaporated to dryness under a nitrogen stream and frozen at -80 °C until LC-MS analysis. |