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MB Sample ID: SA138094
Local Sample ID: | 8-C5-N-HL |
Subject ID: | SU001709 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley |
Age Or Age Range: | 6 weeks-age |
Weight Or Weight Range: | 200 ± 10 |
Gender: | Male |
Animal Animal Supplier: | Vital River Laboratories Co., Ltd (Beijng, China) |
Animal Housing: | 25 ± 2℃, 50±5% relative humidity |
Animal Light Cycle: | 12 h light/12 h dark photoperiod |
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Sample Preparation:
Sampleprep ID: | SP001715 |
Sampleprep Summary: | 100μl of plasma or tissue homogenate was transferred into a 10 ml glass tube and added 20 μl of lipid internal standards. After vortexed for 10 s, 1.5 ml of methanol and 5 ml methyl tert-butyl ether were added to the tube, and the sample was vortexed for 15 min and then added 1.5 ml water, then centrifuged at 4500r/min for 10 min (4 ℃). The upper organic phase was transferred to another glass tube, and the lower water phase was added with 2 ml secondary extractant (methyl tert-butyl ether /methanol/water,10:3:2.5, v/v/v). After vortexed for 10min and centrifuged for 15min at 4500rpm, and the upper organic phase was combined and dried under a gentle nitrogen stream. The residue was re-dissolved in 100 μl methanol/chloroform (1:1, v/v). |