Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA138121

Local Sample ID:	8-A2-PS
Subject ID:	SU001709
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116
Genotype Strain:	Sprague-Dawley
Age Or Age Range:	6 weeks-age
Weight Or Weight Range:	200 ± 10
Gender:	Male
Animal Animal Supplier:	Vital River Laboratories Co., Ltd (Beijng, China)
Animal Housing:	25 ± 2℃, 50±5% relative humidity
Animal Light Cycle:	12 h light/12 h dark photoperiod

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Sample Preparation:

Sampleprep ID:	SP001715
Sampleprep Summary:	100μl of plasma or tissue homogenate was transferred into a 10 ml glass tube and added 20 μl of lipid internal standards. After vortexed for 10 s, 1.5 ml of methanol and 5 ml methyl tert-butyl ether were added to the tube, and the sample was vortexed for 15 min and then added 1.5 ml water, then centrifuged at 4500r/min for 10 min (4 ℃). The upper organic phase was transferred to another glass tube, and the lower water phase was added with 2 ml secondary extractant (methyl tert-butyl ether /methanol/water,10:3:2.5, v/v/v). After vortexed for 10min and centrifuged for 15min at 4500rpm, and the upper organic phase was combined and dried under a gentle nitrogen stream. The residue was re-dissolved in 100 μl methanol/chloroform (1:1, v/v).

Sampleprep ID:

SP001715

Sampleprep Summary:

100μl of plasma or tissue homogenate was transferred into a 10 ml glass tube and added 20 μl of lipid internal standards. After vortexed for 10 s, 1.5 ml of methanol and 5 ml methyl tert-butyl ether were added to the tube, and the sample was vortexed for 15 min and then added 1.5 ml water, then centrifuged at 4500r/min for 10 min (4 ℃). The upper organic phase was transferred to another glass tube, and the lower water phase was added with 2 ml secondary extractant (methyl tert-butyl ether /methanol/water,10:3:2.5, v/v/v). After vortexed for 10min and centrifuged for 15min at 4500rpm, and the upper organic phase was combined and dried under a gentle nitrogen stream. The residue was re-dissolved in 100 μl methanol/chloroform (1:1, v/v).