Return to study ST001680 main page
MB Sample ID: SA154593
Local Sample ID: | anp-neg-Control-417 |
Subject ID: | SU001757 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001763 |
Sampleprep Summary: | The snap frozen liver samples were incubated with 600 µL of 3 mM monobrombimane (MBB) in CH3OH:H2O (80:20) at -20°C for 2 h, followed by 1 h incubation at 0°C. This initial incubation was followed by tissue disruption using stainless steel beads in a TissueLyser (Qiagen) and an additional 30 min at -20°C. Extracts were centrifuged for 15 min at 13000 rpm to pellet insoluble material and supernatants were transferred to clean tubes. This extraction was repeated two additional times and all three supernatants were dried in a speed-vac (Savant) and stored at −80 °C until analysis. |