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MB Sample ID: SA160373
Local Sample ID: | 1204 |
Subject ID: | SU001789 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 6–8 weeks old |
Gender: | Female |
Animal Animal Supplier: | Charles River Frederick Research Model Facility |
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Sample Preparation:
Sampleprep ID: | SP001795 |
Sampleprep Summary: | Blood samples were separated into plasma and packed cells by centrifugation at 3,500g for 15 min at 4°C and stored at -80°C until extraction. 35 µL of plasma were extracted in a water:methanol:chloroform mixture. Centrifuged for 20 min at 4°ᵒC and 13,000 rpm and the resulting upper hydrophilic phase was then transferred to a clean vial and dried under a stream of N2 gas. Dried sediments were resuspended in 60% methanol (aq.) and injected into the LCMS system. |