Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA160379

Local Sample ID:	10cm_4
Subject ID:	SU001790
Subject Type:	Plant
Subject Species:	Panax notoginseng
Taxonomy ID:	44586
Gender:	Not applicable

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Sample Preparation:

Sampleprep ID:	SP001796
Sampleprep Summary:	Approximately 60 mg of frozen powder fibrous roots and 1 mL of methanol (CH3OH) containing 0.5 mg of ribitol were added into a prechilled 2 mL lock-cap centrifuge tube, then vortexed for 10 s. A 300 μL extraction aliquot (H2O:methanol:chloroform=1:2.5:1, v:v:v) was added and ultrasonically extracted for 30 min at 37°C. Then, the sample was centrifuged (1600 g, 3 min) to separate the polar and nonpolar phases. Then the upper polar phase was transferred to a fresh centrifuge tube and added 200 μL sterile water, and then vortexed and centrifuged (1600 g, 4°C for 3 min). A 250 μL aliquot of the upper phase was transferred to a fresh centrifuge tube, dried for 3-4 h at room temperature using a SpeedVac (Christ, Germany). Adding 80 μL of methoxyamine hydrochloride solution (20 mg mL-1 dissolved in pyridine) to each sample and incubating for 90 min at 30°C to protect carbonyl moieties. And then, 40 μL N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) was added and incubating at 37°C for 30 min to trimethylsilylate the acidic protons. After this step, the sample was centrifuged (1600 g, 4°C for 3 min), then the supernatant was stored at 4°C for further analysis.

Sampleprep ID:

SP001796

Sampleprep Summary:

Approximately 60 mg of frozen powder fibrous roots and 1 mL of methanol (CH3OH) containing 0.5 mg of ribitol were added into a prechilled 2 mL lock-cap centrifuge tube, then vortexed for 10 s. A 300 μL extraction aliquot (H2O:methanol:chloroform=1:2.5:1, v:v:v) was added and ultrasonically extracted for 30 min at 37°C. Then, the sample was centrifuged (1600 g, 3 min) to separate the polar and nonpolar phases. Then the upper polar phase was transferred to a fresh centrifuge tube and added 200 μL sterile water, and then vortexed and centrifuged (1600 g, 4°C for 3 min). A 250 μL aliquot of the upper phase was transferred to a fresh centrifuge tube, dried for 3-4 h at room temperature using a SpeedVac (Christ, Germany). Adding 80 μL of methoxyamine hydrochloride solution (20 mg mL-1 dissolved in pyridine) to each sample and incubating for 90 min at 30°C to protect carbonyl moieties. And then, 40 μL N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) was added and incubating at 37°C for 30 min to trimethylsilylate the acidic protons. After this step, the sample was centrifuged (1600 g, 4°C for 3 min), then the supernatant was stored at 4°C for further analysis.