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MB Sample ID: SA163339
Local Sample ID: | NYSM-00489 |
Subject ID: | SU001822 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Long Evans |
Age Or Age Range: | From post-natal day 1 (PN1) to PN80 |
Weight Or Weight Range: | 5g-250g |
Gender: | Male and female |
Animal Animal Supplier: | Envigo |
Animal Housing: | Animal facility at NYU |
Animal Light Cycle: | 7am-7pm |
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Sample Preparation:
Sampleprep ID: | SP001828 |
Sampleprep Summary: | Extraction of samples was performed using a MicroLab STAR automated liquid handling robot (Hamilton Robotics, Inc., Reno, NV, USA); 450 μL of methanol was added to 100 μL of sample to precipitate proteins. Four recovery standards (DL-2-fluorophenylglycine, tridecanoic acid, cholesterol-d6 and 4-chlorophenylalanine) were added to each sample to determine extraction efficiency. To remove proteins, dissociate small molecules bound to protein, and recover metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation (1300 g for 10 min at room temperature). The resultant supernatants were placed in a TurboVap (Zymark) to remove the organic solvent. Each sample extract was then divided into five equal aliquots, dried under nitrogen, and stored in vacuo prior to metabolomic profiling. |