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MB Sample ID: SA167188
Local Sample ID: | HFD_2 |
Subject ID: | SU001873 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6/J |
Age Or Age Range: | 6-7 weeks |
Gender: | Male |
Animal Housing: | temperature-controlled room under specific-pathogen free (SPF) or standard animal housing conditions with free access to food and water |
Animal Feed: | semi-purified normal chow diet (control fat diet (CFD), 7% w/w fat and 16% total energy from fat; AIN93G, Specialty Feeds Pty Ltd, Australia) or high fat diet (HFD, 36% w/w fat and 59% total energy from fat; SF03-002, Specialty Feeds Pty Ltd, Australia) |
Animal Water: | ad libitum |
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Sample Preparation:
Sampleprep ID: | SP001879 |
Sampleprep Summary: | For lipidomics analysis, lipid was extracted from mesenteric lymph by chloroform:methanol (1:3). Samples were vortexed for 1 h at 4°C and then centrifuged at 16,000 g for 10 min. Supernatant was carefully transferred to another tube and stored at -80°C until analysis. Before LC-MS analysis, the extract was dried with nitrogen and reconstituted in 20 µl water and 180 µl butanol-methanol (1:1 v/v). The reconstituted extract was vortexed (Vortex mixer, Ratek) for 200 sec with 20 cycles of 5 sec spin and 20 sec vortex. The extract was sonicated in a water bath for 1 hour which was maintained at <20°C by sonicating the samples on ice. The samples were then centrifuged for 10 min at 16,000 g and the supernatant was transferred to LC-MS vials and stored at 4°C prior to analysis. |
Processing Storage Conditions: | 4? |
Extract Storage: | -80? |