Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA174353

Local Sample ID:	20190525_Indranil_pos_spme_meta8_1
Subject ID:	SU001938
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Female

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Sample Preparation:

Sampleprep ID:	SP001944
Sampleprep Summary:	Each cell pellet was thawed on ice and resuspended in 500 μL ice-cold water by vortexing for 3 seconds and 500 μL of chilled (–80°C) 90% methanol + 10% chloroform solution was immediately added and vortexed for another 10 seconds and then kept on ice. Samples were incubated for 30 minutes at 4°C while rotating and then centrifuged at 800×g for 10 mins at 4°C. The supernatants were transferred to fresh tubes and centrifuged at 16000×g for 45 minutes at 4°C. The cleared supernatant containing metabolites were cleaned using a SPME (solid phase microextraction) protocol adopted from Mousavi et. al. (Mousavi et al., 2019), vacufuged to dryness and stored at –80°C. The cell pellets were used for protein extraction using GuHCl lysis method as described below.

Sampleprep ID:

SP001944

Sampleprep Summary:

Each cell pellet was thawed on ice and resuspended in 500 μL ice-cold water by vortexing for 3 seconds and 500 μL of chilled (–80°C) 90% methanol + 10% chloroform solution was immediately added and vortexed for another 10 seconds and then kept on ice. Samples were incubated for 30 minutes at 4°C while rotating and then centrifuged at 800×g for 10 mins at 4°C. The supernatants were transferred to fresh tubes and centrifuged at 16000×g for 45 minutes at 4°C. The cleared supernatant containing metabolites were cleaned using a SPME (solid phase microextraction) protocol adopted from Mousavi et. al. (Mousavi et al., 2019), vacufuged to dryness and stored at –80°C. The cell pellets were used for protein extraction using GuHCl lysis method as described below.