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MB Sample ID: SA177707

Local Sample ID:	KO_ZT22_1
Subject ID:	SU001993
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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MS

Sample Preparation:

Sampleprep ID:	SP001999
Sampleprep Summary:	Heart ventricle tissues were harvested from male mice at 3 months old (n = 3 at each condition) and were snap-frozen in liquid nitrogen. Lipids were extracted as previously described 55–57. Lipids were separated on a Shimadzu CTO-20A Nexera X2 UHPLC systems, 1.8 μm particle 50 × 2.1 mm Acquity HSS UPLC T3 column (Waters, Milford, MA). Lipidomics acquisition performed in both pos and neg mode ionization. Pos mode lipids were normalized by Internal Standard PC 17:0; [M+H]+, Neg mode lipids were normalized by Internal Standard PC 34:0.

Sampleprep ID:

SP001999

Sampleprep Summary:

Heart ventricle tissues were harvested from male mice at 3 months old (n = 3 at each condition) and were snap-frozen in liquid nitrogen. Lipids were extracted as previously described 55–57. Lipids were separated on a Shimadzu CTO-20A Nexera X2 UHPLC systems, 1.8 μm particle 50 × 2.1 mm Acquity HSS UPLC T3 column (Waters, Milford, MA). Lipidomics acquisition performed in both pos and neg mode ionization. Pos mode lipids were normalized by Internal Standard PC 17:0; [M+H]+, Neg mode lipids were normalized by Internal Standard PC 34:0.