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MB Sample ID: SA177709
Local Sample ID: | WT_ZT10_1 |
Subject ID: | SU001993 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP001999 |
Sampleprep Summary: | Heart ventricle tissues were harvested from male mice at 3 months old (n = 3 at each condition) and were snap-frozen in liquid nitrogen. Lipids were extracted as previously described 55–57. Lipids were separated on a Shimadzu CTO-20A Nexera X2 UHPLC systems, 1.8 μm particle 50 × 2.1 mm Acquity HSS UPLC T3 column (Waters, Milford, MA). Lipidomics acquisition performed in both pos and neg mode ionization. Pos mode lipids were normalized by Internal Standard PC 17:0; [M+H]+, Neg mode lipids were normalized by Internal Standard PC 34:0. |