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MB Sample ID: SA178386

Local Sample ID:	tube.90
Subject ID:	SU002004
Subject Type:	Invertebrate
Subject Species:	Drosophila melanogaster
Taxonomy ID:	7227
Gender:	Male and female

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Sample Preparation:

Sampleprep ID:	SP002010
Sampleprep Summary:	Metabolites were extracted by homogenizing samples in 200uL HPLC water (Sigma) using a TissueLyser (TissueLyser II, Qiagen) for 6 minutes at 25 Hz at 4°C. We then added 800uL methanol and incubated for 30 minutes on dry ice. The suspension was homogenized again for 10 minutes at 25Hz, then spun at 14,000rcf for 10 minutes at 4°C. The supernatant was transferred to a new tube and dried in a Speed-Vac at 30°C.

Sampleprep ID:

SP002010

Sampleprep Summary:

Metabolites were extracted by homogenizing samples in 200uL HPLC water (Sigma) using a TissueLyser (TissueLyser II, Qiagen) for 6 minutes at 25 Hz at 4°C. We then added 800uL methanol and incubated for 30 minutes on dry ice. The suspension was homogenized again for 10 minutes at 25Hz, then spun at 14,000rcf for 10 minutes at 4°C. The supernatant was transferred to a new tube and dried in a Speed-Vac at 30°C.