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MB Sample ID: SA181878
Local Sample ID: | HFD-HPVB-3 |
Subject ID: | SU002013 |
Subject Type: | Mammal |
Subject Species: | Macaca fascicularis |
Taxonomy ID: | 9541 |
Age Or Age Range: | 16.3 - 18.8 yo |
Weight Or Weight Range: | 5.73 - 10.56 kg |
Gender: | Male |
Animal Animal Supplier: | Guangzhou Huazhen Biosciences Co., Ltd, Guangzhou |
Animal Housing: | Guangzhou Huazhen Biosciences Co., Ltd, Guangzhou |
Animal Light Cycle: | 12-h/12-h light-dark cycle |
Animal Feed: | normal food (19.26 % protein, 5% fat, no sugar, and no cholesterol), or high-fat and high-sugar (HFHS) food (≥ 30% sugar, ≥ 15% fat, ≥ 10.5% protein, and ≥ 0.5% cholesterol) |
Animal Inclusion Criteria: | spontaneous diabetes mellitus and HFHS food-fed |
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Sample Preparation:
Sampleprep ID: | SP002019 |
Sampleprep Summary: | The hydrophilic and hydrophobic compounds were extracted using methanol/water and MTBE/methanol/water solvent systems, respectively. Samples were first thawed on ice. To extract hydrophilic metabolites from the tissue samples, 1 ml of methanol/water (7:3, v/v) was added to 50 mg of the liver, and homogenized with steel balls for 3 min at 30 Hz, followed by 1 min of a vortex. The homogenate was then centrifuged at 12,000 rpm for 10 min at 4°C to collect the supernatant. Hydrophobic compounds were extracted from another 50 mg using a slightly modified protocol. Homogenization was done with 1 ml of MTBE/methanol (10:3, v/v) and 100 µl of water was mixed with the homogenate to extract before centrifugation. For the sera of PB and HPVB, 3 volumes (v/v) of methanol and a mixture of MTBE and methanol (10:3, v/v) were whirled with the serum samples for 3 min, followed by centrifugation at 12,000 rpm for 10 min at 4°C. All collected supernatants were dried and store at -80°C until LC-MS/MS analysis. Internal standards were dissolved in the solvents before extraction. |