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MB Sample ID: SA182125

Local Sample ID:SH48
Subject ID:SU002015
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Sample Preparation:

Sampleprep ID:SP002021
Sampleprep Summary:To undertake comprehensive lipidomic profiling, a sample preparation approach for serum based on liquid-liquid MTBE extraction was utilized to cover diverse classes of lipids. Untargeted lipidomic analysis was performed using a Dionex UltiMate 3000 UHPLC system (Santa Clara, CA, USA) linked online via electrospray ionization source (ESI) with a Q ExactiveTM Hybrid Quadrupole-OrbitrapTM Mass Spectrometer (Thermo Fisher Scientific, Inc., MA, USA). Comprehensive metabolomic analysis was performed on a TRACE 1310 gas chromatograph equipped with an AS 1310 autosampler connected to a TSQ 8000 triple quadrupole mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) as described previously 14.
Sampleprep Protocol ID:346 Lipidomics_LCMSProtocol.pdf
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