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MB Sample ID: SA182407
| Local Sample ID: | WH35 |
| Subject ID: | SU002015 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP002021 |
| Sampleprep Summary: | To undertake comprehensive lipidomic profiling, a sample preparation approach for serum based on liquid-liquid MTBE extraction was utilized to cover diverse classes of lipids. Untargeted lipidomic analysis was performed using a Dionex UltiMate 3000 UHPLC system (Santa Clara, CA, USA) linked online via electrospray ionization source (ESI) with a Q ExactiveTM Hybrid Quadrupole-OrbitrapTM Mass Spectrometer (Thermo Fisher Scientific, Inc., MA, USA). Comprehensive metabolomic analysis was performed on a TRACE 1310 gas chromatograph equipped with an AS 1310 autosampler connected to a TSQ 8000 triple quadrupole mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) as described previously 14. |
| Sampleprep Protocol ID: | 346 Lipidomics_LCMSProtocol.pdf |
