Metabolomics Workbench : NIH Data Repository

Return to study ST001945 main page

MB Sample ID: SA183494

Local Sample ID:	10_1H_09Abril2018
Subject ID:	SU002023
Subject Type:	Mammal
Subject Species:	Hydrochoerus hydrochaeris
Gender:	Female

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP002029
Sampleprep Summary:	Approximately 30 mg of dry cecal and rectal contents, and 300 μL of solution 2:1 (methanol: chloroform) were mixed and sonicated for 1 min (4 cycles of 15 sec with intervals of 10 sec) and placed at 4 °C for 15 min. Next, 300 μL of solution 1:1 (methanol: ultrapure water) was added, followed by centrifugation at 16,000 g and 4 °C for 20 min. The supernatant was transferred to a new tube and were dried in CentriVap Solvent System (Labconco Corporation). Samples were diluted to 630 μL by addition of D2O, 70 μL of sodium phosphate buffer (final concentration 0.1 M) containing dimethyl-silapentane-sulfonate (final concentration 0.5 mM) for NMR chemical shift reference and concentration calibration. The samples were filtrated in a syringe filter with a 0.22 µm pore size hydrophilic polyethersulfone (PES) membrane.

Sampleprep ID:

SP002029

Sampleprep Summary:

Approximately 30 mg of dry cecal and rectal contents, and 300 μL of solution 2:1 (methanol: chloroform) were mixed and sonicated for 1 min (4 cycles of 15 sec with intervals of 10 sec) and placed at 4 °C for 15 min. Next, 300 μL of solution 1:1 (methanol: ultrapure water) was added, followed by centrifugation at 16,000 g and 4 °C for 20 min. The supernatant was transferred to a new tube and were dried in CentriVap Solvent System (Labconco Corporation). Samples were diluted to 630 μL by addition of D2O, 70 μL of sodium phosphate buffer (final concentration 0.1 M) containing dimethyl-silapentane-sulfonate (final concentration 0.5 mM) for NMR chemical shift reference and concentration calibration. The samples were filtrated in a syringe filter with a 0.22 µm pore size hydrophilic polyethersulfone (PES) membrane.