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MB Sample ID: SA183523

Local Sample ID:	D01
Subject ID:	SU002024
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	19-79
Weight Or Weight Range:	49-93
Height Or Height Range:	1.5-1.9
Gender:	Male and female
Human Ethnicity:	Brazilian
Human Medications:	BD patients used a wide range of different medications, mostly mood stabilizers and antipsychotics
Human Inclusion Criteria:	Controls: Any adult over 18 years respecting the excusion criteria / BD: Any adult over 18 years old with BD diagnosed by a psychiatrist, respecting exclusion criteria psychiat
Human Exclusion Criteria:	Controls : negative history of any psychiatric disorder or use of any kind of psychiatric medication / BD : concomitant diseases such as AIDS, hepatitis, endocrinal or metabolic diseases, substance use (except nicotine dependence), and pregnancy or postpartum period

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Sample Preparation:

Sampleprep ID:	SP002030
Sampleprep Summary:	The metabolites from serum samples were extracted by a simple protein precipitation method, using 50 µL of serum and 150 µL of MeOH and 150 µL of pentadecanoic acid as the internal standard. The mixture was vortexed, centrifuged at 15000 RPM, subsequently separating and discarding the protein phase. The aqueous phase was then dried in a SpeedVac at room temp and the dried samples were then submitted to a two-step derivatization, where 10 µL of methoxamine hydrochloride pyridine solution (40 mg mL-1) was added to the sample, kept at 30 ºC for 90 minutes. After the first derivatization step, 90 µL of MSTFA with 1 % (v/v) of TMCS (trimethylchlorosilane) was added, with the sample being kept at 37 ºC for 30 minutes.

Sampleprep ID:

SP002030

Sampleprep Summary:

The metabolites from serum samples were extracted by a simple protein precipitation method, using 50 µL of serum and 150 µL of MeOH and 150 µL of pentadecanoic acid as the internal standard. The mixture was vortexed, centrifuged at 15000 RPM, subsequently separating and discarding the protein phase. The aqueous phase was then dried in a SpeedVac at room temp and the dried samples were then submitted to a two-step derivatization, where 10 µL of methoxamine hydrochloride pyridine solution (40 mg mL-1) was added to the sample, kept at 30 ºC for 90 minutes. After the first derivatization step, 90 µL of MSTFA with 1 % (v/v) of TMCS (trimethylchlorosilane) was added, with the sample being kept at 37 ºC for 30 minutes.