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MB Sample ID: SA184587
| Local Sample ID: | differentiation-2 |
| Subject ID: | SU002038 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 51.3±5 |
| Gender: | Female |
| Cell Biosource Or Supplier: | Promocell (GmbH, Heidelberg, Germany) and Zen-Bio (Zen-Bio, Inc., Research Triangle Park, NC, USA) |
| Cell Strain Details: | Human subcutaneous white adipocytes |
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Sample Preparation:
| Sampleprep ID: | SP002044 |
| Sampleprep Summary: | The cell suspension was transferred to a tube and centrifuged at 220g for 3 min, the supernatant was discarded and the cells were suspended in 100 μL of Milli-Q water. Each sample was normalized by measuring the amount of protein by bicinchoninic acid (BCA) protein assay using Pierce Micro BCA Protein Assay Kit (Pierce, Rockford, IL, USA) and the final volume of 600 μL was adjusted using water.10 μL of Splash Lipidomix internal standards (Avanti, Alabaster, AL, USA) was added and the Bligh and Dyer method was used for lipid extraction. |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | -20℃ |
